Journal: bioRxiv
Article Title: Investigating the Impact of Hypoxia and Syncytialization on Lipid Nanoparticle-Mediated mRNA Delivery to Placental Cells
doi: 10.1101/2025.06.23.661191
Figure Lengend Snippet: Cellular response to culture in under hypoxic (1% O2) conditions. (A) HIF-1α expression in cells and (B) PlGF secretion from cells cultured in normoxia (21% O2) or hypoxia (1% O2) at 24, 48, and 72 hours. “ND” is not detectable. *p<0.05, ***p<0.001 and ****p<0.0001 by two-way ANOVA with post hoc Tukey. (C) HTR8, (D) JAR, and (E) BeWo growth curves during cell culture in normoxia and hypoxia over time. ***p<0.001 and ****p<0.0001 by Analysis of Covariance (ANCOVA). Additional statistical analyses are provided in Tables S8-S11.
Article Snippet: For experiments using low oxygen cultures (termed “hypoxia” herein), a hypoxic cell culture chamber (StemCell Technologies) was purged with low oxygen gas (1% O2, 5% CO2, and 94% N2) for 5 minutes.
Techniques: Expressing, Cell Culture